ジャーナル
/
免疫学と感染
/
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

DOI:
10.3791/56866-v

•

08:50 min

•

March 14, 2018

•

, , , , , , , ,
1Center for Metabolic and Vascular Biology,Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences,Arizona State University, 3物理学科,Arizona State University, 4Center for Biological Physics,Arizona State University, 5Biodesign Institute,Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences,University of Notre Dame, 7Advanced Imaging Center,HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility,European Molecular Biology Laboratory

章

  • 00:05標題
  • 00:47Preparing Acid-cleaned Cover Glass
  • 01:59Preparing of Fusogenic Optical-quality Surfaces
  • 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
  • 03:58Fabricating Glass Bottom Dishes
  • 04:55Collecting Thioglycollate-elicited Macrophages
  • 06:15Results: Macrophage Fusion on Glass Surfaces
  • 07:51Conclusion

概要

自動翻訳

自動翻訳

This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.

タグ

Optical GlassMacrophage FusionMicroscopyAcid CleaningParaffin CoatingGold TEM Grid

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Glass Wool Filters for Concentrating Waterborne Viruses and Agricultural Zoonotic Pathogens

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Bone Marrow-derived Macrophage Production

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

Read Article