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Mapeo metabólico: Citoquímica enzimática cuantitativa y la histoquímica para determinar la actividad de Deshidrogenasas en células y tejidos
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
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Mapeo metabólico: Citoquímica enzimática cuantitativa y la histoquímica para determinar la actividad de Deshidrogenasas en células y tejidos

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11,253 Views

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08:36 min

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May 26, 2018

DOI:

10.3791/56843-v

DOI:
10.3791/56843-v

•

08:36 min
May 26, 2018

•

6 Views
, , ,
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Summary

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Aquí, presentamos un protocolo que puede utilizarse para visualizar microscópicamente y cuantificar la actividad de Deshidrogenasas en las células y tejidos y su cinética, función y localización subcelular.

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Cite this Article

Molenaar, R. J., Khurshed, M., Hira, V. V., Van Noorden, C. J. Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues. J. Vis. Exp. (135), e56843, doi:10.3791/56843 (2018).

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