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Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

DOI:
10.3791/56843-v

•

08:36 min

•

May 26, 2018

•

, , ,
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Bölümler

  • 00:00Başlık
  • 00:50Preparing Reaction Buffer
  • 03:50Applying Incubation Medium to Tissue Sections
  • 05:32Washing the Microscopy Slides
  • 06:33Enclose the Tissue Sections on Microscopy Slides
  • 07:13Representative Results
  • 08:14Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.

Etiketler

Metabolic MappingEnzyme CytochemistryHistochemistryDehydrogenase ActivityCellsPolyvinyl AlcoholPVAPhosphate BufferNitroblue Tetrazolium ChlorideNitroBTEthanolDimethylformamideReaction BufferCytospinsMetabolizmaCancer CellsTherapeutic Target

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