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代謝のマッピング: 定量的酵素組織化学および組織化学細胞や組織における脱水素酵素の活性を決定するには
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
Please note that all translations are automatically generated. Click here for the English version.

代謝のマッピング: 定量的酵素組織化学および組織化学細胞や組織における脱水素酵素の活性を決定するには

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11,253 Views

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08:36 min

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May 26, 2018

DOI:

10.3791/56843-v

DOI:
10.3791/56843-v

•

08:36 min
May 26, 2018

•

6 Views
, , ,
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Summary

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ここでは、顕微鏡可視化し、細胞と組織と反応速度論、関数と内局内脱水素酵素の活動を定量化に使用できるプロトコルを提案する.

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Molenaar, R. J., Khurshed, M., Hira, V. V., Van Noorden, C. J. Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues. J. Vis. Exp. (135), e56843, doi:10.3791/56843 (2018).

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