Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.
Molenaar, R. J., Khurshed, M., Hira, V. V., Van Noorden, C. J. Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues. J. Vis. Exp. (135), e56843, doi:10.3791/56843 (2018).