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Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

DOI:
10.3791/56843-v

•

08:36 min

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May 26, 2018

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, , ,
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Chapters

  • 00:00Title
  • 00:50Preparing Reaction Buffer
  • 03:50Applying Incubation Medium to Tissue Sections
  • 05:32Washing the Microscopy Slides
  • 06:33Enclose the Tissue Sections on Microscopy Slides
  • 07:13Representative Results
  • 08:14Conclusion

Summary

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자동 번역

Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.

Tags

Metabolic MappingEnzyme CytochemistryHistochemistryDehydrogenase ActivityCellsPolyvinyl AlcoholPVAPhosphate BufferNitroblue Tetrazolium ChlorideNitroBTEthanolDimethylformamideReaction BufferCytospinsMetabolismCancer CellsTherapeutic Target

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