Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Remco J. Molenaar; Mohammed Khurshed; Vashendriya V.V. Hira; Cornelis J.F. Van Noorden

doi:10.3791/56843

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Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

11,205 Views

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08:36 min

•

May 26, 2018

DOI:

10.3791/56843-v

DOI:

10.3791/56843-v

•

08:36 min

May 26, 2018

•

6 Views

Remco J. Molenaar¹, Mohammed Khurshed¹, Vashendriya V.V. Hira¹, Cornelis J.F. Van Noorden¹

¹Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

概要

Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.

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Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

概要

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