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Journal
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Biochemistry
/
磁気ピンセットで G quadruplexes の単一分子マニピュレーション
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Biochemistry
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JoVE Journal
Biochemistry
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
Please note that all translations are automatically generated.
Click here for the English version.
磁気ピンセットで G quadruplexes の単一分子マニピュレーション
DOI:
10.3791/56328-v
•
08:28 min
•
September 19, 2017
•
Huijuan You*
1
,
Shimin Le*
2,3
,
Hu Chen
4
,
Linyan Qin
,
Jie Yan
4,5
1
School of Pharmacy, Tongji Medical College
,
Huazhong University of Science and Technology
,
2
Mechanobiology Institute
,
National University of Singapore
,
3
Department of Physics
,
National University of Singapore
,
4
Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics
,
Xiamen University
,
5
Center for Bioimaging Sciences
,
National University of Singapore
Chapters
00:05
Title
01:10
Magnetic Tweezers Setup and Identification of Single dsDNA Tether
04:43
Single-molecule Manipulation of G4 on the Presence and Absence of Binding Proteins
05:57
Results: Single-molecule Manipulation of a G4 and Force-jump Measurements of G4 Unfolding
07:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
G quadruplexes を操作する単一分子磁気ピンセット プラットフォームによって報告されます、G4 の安定性と制御の研究によって、様々 なタンパク質。
Tags
G-quadruplex
Magnetic Tweezers
Single-molecule Manipulation
Folding And Unfolding
Binding Proteins
DNA-protein Interactions
Helicase Activity
DNA Tether Formation
Paramagnetic Beads
Nanometer-scale Dynamics
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