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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

5,989 Views

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08:29 min

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April 19, 2019

DOI:

10.3791/59199-v

DOI:

10.3791/59199-v

•

08:29 min

April 19, 2019

•

2 Views

Simon Leclerc^1,2, Youri Arntz², Yuichi Taniguchi^1,3

¹Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research,RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie,INSERM, ³PRESTO, Japan Science and Technology Agency

Summary

Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.

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