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Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
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Biochemistry
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JoVE Journal Biochemistry
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

English

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9,852 Views

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11:22 min

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January 30, 2018

DOI:

10.3791/56896-v

DOI:
10.3791/56896-v

•

11:22 min
January 30, 2018

•

5 Views
, , ,
1Institute of Physical Chemistry,University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Summary

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Here, we present a protocol to obtain three-color smFRET data and its analysis with a 3D ensemble Hidden Markov Model. With this approach, scientists can extract kinetic information from complex protein systems, including cooperativity or correlated interactions.

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Cite this Article

Götz, M., Wortmann, P., Schmid, S., Hugel, T. Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions. J. Vis. Exp. (131), e56896, doi:10.3791/56896 (2018).

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