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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
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Biochemistry
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JoVE Journal Biochemistry
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

2,982 Views

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06:26 min

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September 20, 2020

DOI:

10.3791/61680-v

DOI:
10.3791/61680-v

•

06:26 min
September 20, 2020

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, , , ,
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology,Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology,Texas A&M University, 4Department of Pharmacology and Chemical Biology,Baylor College of Medicine

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Single Cell AnalysisTranscriptionally Active AllelesSingle Molecule FISHRNA QuantificationGene TranscriptionEstrogen Receptor AgonistRNA FISHGREB1 IntronGREB1 ExonDAPIFluorescence Microscopy

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Mistry, R. M., Singh, P. K., Mancini, M. G., Stossi, F., Mancini, M. A. Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH. J. Vis. Exp. (163), e61680, doi:10.3791/61680 (2020).

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