Journal
/
Biochemistry
/
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

English

Automatically Generated

6,547 Views

•

08:34 min

•

February 05, 2020

DOI:

10.3791/60774-v

DOI:
10.3791/60774-v

•

08:34 min
February 05, 2020

•

4 Views
, , , , , ,
1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering,Nanjing University

Summary

Automatically generated

Here, we present a protocol to conjugate protein monomer by enzymes forming protein polymer with a controlled sequence and immobilize it on the surface for single-molecule force spectroscopy studies.

Explore More Videos

OaAEP1Enzymatic SynthesisPolymerized ProteinSingle-molecule Force SpectroscopyProtein OligomerPolymerization DegreeProtein-based MaterialCysteinePotassium ChromateSulfuric AcidAPTESSulfo-SMCCGL-ELP-50 Cys-proteinCantilever Surface

Read Article

Cite this Article

Deng, Y., Zheng, B., Liu, Y., Shi, S., Nie, J., Wu, T., Zheng, P. OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy. J. Vis. Exp. (156), e60774, doi:10.3791/60774 (2020).

Download .ris file

Copy

Share Video

.

Copy