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Dirigida a tioles de la cisteína para in Vitro específica glicosilación de proteínas recombinantes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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Dirigida a tioles de la cisteína para in Vitro específica glicosilación de proteínas recombinantes

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6,465 Views

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11:25 min

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October 04, 2017

DOI:

10.3791/56302-v

DOI:
10.3791/56302-v

•

11:25 min
October 04, 2017

•

2 Views
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1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario

Summary

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Análisis bioquímicos y estructurales de proteínas glicosiladas requieren cantidades relativamente grandes de muestras homogéneas. Aquí, presentamos un método químico eficiente para el glycosylation sitio-específico de proteínas recombinantes purificados de bacterias dirigiéndose a tioles reactivos de Cys.

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Cite this Article

Choi, Y. J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q., Stathopulos, P. B. Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins. J. Vis. Exp. (128), e56302, doi:10.3791/56302 (2017).

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