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組換えタンパク質の部位特異的糖体外のシステインのチオール基を対象と
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Immunology and Infection
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JoVE Journal Immunology and Infection
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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組換えタンパク質の部位特異的糖体外のシステインのチオール基を対象と

日本語

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6,468 Views

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11:25 min

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October 04, 2017

DOI:

10.3791/56302-v

DOI:
10.3791/56302-v

•

11:25 min
October 04, 2017

•

2 Views
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1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario

Summary

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糖タンパク質の生化学的および構造解析では、均質な試料の比較的大量を必要とします。反応システイン チオール化合物をターゲットに細菌から精製組換えタンパク質の部位特異的糖鎖の効率的な化学的方法を紹介します。

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Cite this Article

Choi, Y. J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q., Stathopulos, P. B. Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins. J. Vis. Exp. (128), e56302, doi:10.3791/56302 (2017).

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