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Targeting Cystein Thiole für in-vitro- standortspezifische Glykosylierung von rekombinanten Proteinen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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Targeting Cystein Thiole für in-vitro- standortspezifische Glykosylierung von rekombinanten Proteinen

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6,468 Views

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11:25 min

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October 04, 2017

DOI:

10.3791/56302-v

DOI:
10.3791/56302-v

•

11:25 min
October 04, 2017

•

2 Views
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1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario

Summary

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Biochemische und strukturelle Analysen des glykosylierten Proteinen erfordern relativ große Mengen an homogenen Proben. Hier präsentieren wir Ihnen eine effiziente chemische Methode für standortspezifische Glykosylierung von rekombinanten Proteinen durch gezielte reaktive Cys Thiole von Bakterien gereinigt.

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Cite this Article

Choi, Y. J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q., Stathopulos, P. B. Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins. J. Vis. Exp. (128), e56302, doi:10.3791/56302 (2017).

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