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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

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11:25 min

October 04, 2017

DOI:

11:25 min
October 04, 2017

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Summary

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Biochemical and structural analyses of glycosylated proteins require relatively large amounts of homogeneous samples. Here, we present an efficient chemical method for site-specific glycosylation of recombinant proteins purified from bacteria by targeting reactive Cys thiols.

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