/
/
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
6,468 Views
•
11:25 min
•
October 04, 2017
DOI:
Summary
Automatically generated
Biochemical and structural analyses of glycosylated proteins require relatively large amounts of homogeneous samples. Here, we present an efficient chemical method for site-specific glycosylation of recombinant proteins purified from bacteria by targeting reactive Cys thiols.
Read Article
Cite this Article
Choi, Y. J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q., Stathopulos, P. B. Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins. J. Vis. Exp. (128), e56302, doi:10.3791/56302 (2017).
Copy