/
/
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
6,468 Views
•
11:25 min
•
October 04, 2017
DOI:
Summary
Automatically generated
Biochemical and structural analyses of glycosylated proteins require relatively large amounts of homogeneous samples. Here, we present an efficient chemical method for site-specific glycosylation of recombinant proteins purified from bacteria by targeting reactive Cys thiols.
Read Article
Cite this Article
Choi, Y. J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q., Stathopulos, P. B. Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins. J. Vis. Exp. (128), e56302, doi:10.3791/56302 (2017).
Copy