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Cysteïne thiolen richtend voor in Vitro site-specifieke glycosylatie van recombinante eiwitten
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Immunology and Infection
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JoVE Journal Immunology and Infection
Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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Cysteïne thiolen richtend voor in Vitro site-specifieke glycosylatie van recombinante eiwitten

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6,468 Views

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11:25 min

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October 04, 2017

DOI:

10.3791/56302-v

DOI:
10.3791/56302-v

•

11:25 min
October 04, 2017

•

2 Views
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1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry,University of Western Ontario

Summary

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Biochemische en structurele analyses van geglycosyleerde eiwitten vereist relatief grote hoeveelheden van homogene monsters. Hier presenteren we een efficiënte chemische methode voor site-specifieke glycosylatie van recombinante eiwitten gezuiverd van bacteriën door zich te richten van reactieve Cys thiolen.

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Choi, Y. J., Zhu, J., Chung, S., Siddiqui, N., Feng, Q., Stathopulos, P. B. Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins. J. Vis. Exp. (128), e56302, doi:10.3791/56302 (2017).

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