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Developmental Biology
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水平整体安装:厚三维组织横截面皮肤的新型加工和成像方案
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
Please note that all translations are automatically generated.
Click here for the English version.
水平整体安装:厚三维组织横截面皮肤的新型加工和成像方案
DOI:
10.3791/56106-v
•
08:31 min
•
August 02, 2017
•
Lucia Salz
2
,
Ryan R. Driskell
2
1
Centre for Stem Cells and Regenerative Medicine
,
King’s College London
,
2
School of Molecular Biosciences
,
Washington State University
Chapters
00:05
Title
00:55
Preparation of Skin Samples
02:07
Cryosectioning
03:17
Immunofluorescent Labeling
05:05
Mounting for Microscopic Visualization
06:36
Results: Horizontal Whole Mount Compared to a Classical Cryosection
07:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作提出了一种新颖的加工和成像协议,用于厚的三维组织横截面分析,可以充分利用共聚焦成像模式。该协议保留抗原性,并代表一个强大的系统来分析皮肤组织学和潜在的其他组织类型。
Tags
Horizontal Whole Mount
Skin
Imaging Protocol
Confocal Microscopy
Cryostat Sectioning
Immunofluorescent Labeling
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