Journal
/
Developmental Biology
/
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

English

Automatically Generated

12,690 Views

•

08:42 min

•

March 29, 2018

DOI:

10.3791/57406-v

DOI:
10.3791/57406-v

•

08:42 min
March 29, 2018

•

6 Views
, ,
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Summary

Automatically generated

Here, we describe a high resolution whole-mount imaging method in the entire adult mouse ear skin, which enables us to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels, as well as immune cell distribution.

Explore More Videos

Whole-mountConfocal MicroscopyEar SkinNeuro-vascular BranchingMorphogenesisImmune Cell DistributionPeripheral NervesBlood VesselsImmunohistochemistrySkin Preparation3D VisualizationNormal And Pathological Conditions

Read Article

Cite this Article

Yamazaki, T., Li, W., Mukouyama, Y. Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution. J. Vis. Exp. (133), e57406, doi:10.3791/57406 (2018).

Download .ris file

Copy

Share Video

.

Copy