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Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

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7,859 Views

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09:33 min

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November 18, 2017

DOI:

10.3791/56261-v

DOI:
10.3791/56261-v

•

09:33 min
November 18, 2017

•

2 Views
, , , ,
1Department of Biological Sciences,University of Notre Dame

Summary

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Cilia development is vital to proper organogenesis. This protocol describes an optimized method to label and visualize ciliated cells of the zebrafish.

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Cite this Article

Marra, A. N., Ulrich, M., White, A., Springer, M., Wingert, R. A. Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence. J. Vis. Exp. (129), e56261, doi:10.3791/56261 (2017).

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