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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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Developmental Biology
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JoVE Journal Developmental Biology
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

English

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9,736 Views

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06:17 min

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August 25, 2018

DOI:

10.3791/57784-v

DOI:
10.3791/57784-v

•

06:17 min
August 25, 2018

•

10 Views
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1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology,Qilu Hospital of Shandong University

Summary

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Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

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Cite this Article

Liu, Z., Wen, J., Leng, X., Zhou, Q., Zhou, C., Zhao, H., Wu, X. A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue. J. Vis. Exp. (138), e57784, doi:10.3791/57784 (2018).

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