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Generación de variantes de Escape de neutralizar anticuerpos Monoclonal del Virus de la Influenza
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
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Generación de variantes de Escape de neutralizar anticuerpos Monoclonal del Virus de la Influenza

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11,281 Views

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07:55 min

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August 29, 2017

DOI:

10.3791/56067-v

DOI:
10.3791/56067-v

•

07:55 min
August 29, 2017

•

3 Views
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1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

Summary

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Se describe un método por el cual identificamos residuos críticos necesarios para la Unión de humanos o murinos anticuerpos monoclonales dirigidos a la hemaglutinina viral del virus de la gripe A. El protocolo puede ser adaptado a otras glicoproteínas de superficie del virus y sus correspondientes anticuerpos neutralizantes.

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Cite this Article

Leon, P. E., Wohlbold, T. J., He, W., Bailey, M. J., Henry, C. J., Wilson, P. C., Krammer, F., Tan, G. S. Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies. J. Vis. Exp. (126), e56067, doi:10.3791/56067 (2017).

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