Journal
/
Immunology and Infection
/
Generatie van Escape varianten van het Influenza Virus monoklonale antistoffen neutraliseren
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Please note that all translations are automatically generated. Click here for the English version.

Generatie van Escape varianten van het Influenza Virus monoklonale antistoffen neutraliseren

Nederlands

Automatically Generated

11,281 Views

•

07:55 min

•

August 29, 2017

DOI:

10.3791/56067-v

DOI:
10.3791/56067-v

•

07:55 min
August 29, 2017

•

3 Views
, , , , , , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

Summary

Automatically generated

Beschrijven we een methode waarmee we identificeren kritische residuen nodig zijn voor de binding van de mens of lymfkliertest monoclonal antilichamen die gericht zijn op de virale hemagglutinine van influenza A-virussen. Het protocol kan worden aangepast aan andere oppervlakte glycoproteïnen van virus en hun overeenkomstige neutraliserende antilichamen.

Explore More Videos

Neutralizing AntibodiesMonoclonal AntibodiesEpitope MappingVirus Escape VariantsHemagglutination Inhibition (HI) AssayVirus NeutralizationEmbryonated Chicken EggsMDCK CellsVirus PassagingVirus-antibody Interactions

Read Article

Cite this Article

Leon, P. E., Wohlbold, T. J., He, W., Bailey, M. J., Henry, C. J., Wilson, P. C., Krammer, F., Tan, G. S. Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies. J. Vis. Exp. (126), e56067, doi:10.3791/56067 (2017).

Download .ris file

Copy

Share Video

.

Copy