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Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

11,281 Views

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07:55 min

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August 29, 2017

DOI:

10.3791/56067-v

DOI:
10.3791/56067-v

•

07:55 min
August 29, 2017

•

3 Views
, , , , , , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

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We describe a method by which we identify critical residues required for the binding of human or murine monoclonal antibodies that target the viral hemagglutinin of influenza A viruses. The protocol can be adapted to other virus surface glycoproteins and their corresponding neutralizing antibodies.

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Leon, P. E., Wohlbold, T. J., He, W., Bailey, M. J., Henry, C. J., Wilson, P. C., Krammer, F., Tan, G. S. Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies. J. Vis. Exp. (126), e56067, doi:10.3791/56067 (2017).

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