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Generation von Escape Varianten des Influenza-Virus Monoclonal Antikörper neutralisieren
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
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Generation von Escape Varianten des Influenza-Virus Monoclonal Antikörper neutralisieren

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11,281 Views

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07:55 min

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August 29, 2017

DOI:

10.3791/56067-v

DOI:
10.3791/56067-v

•

07:55 min
August 29, 2017

•

3 Views
, , , , , , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

Summary

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Wir beschreiben eine Methode mit der identifizieren wir kritische Rückstände für die Bindung von menschlichen oder murinen monoklonalen Antikörpern, die die virale Hämagglutinin der Influenza A-Viren Zielen erforderlich. Das Protokoll kann zu anderen Virus Oberfläche Glykoproteine und ihre entsprechenden neutralisierende Antikörper angepasst werden.

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Cite this Article

Leon, P. E., Wohlbold, T. J., He, W., Bailey, M. J., Henry, C. J., Wilson, P. C., Krammer, F., Tan, G. S. Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies. J. Vis. Exp. (126), e56067, doi:10.3791/56067 (2017).

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