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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
JoVE Revista
Genética
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JoVE Revista Genética
Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

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05:34 min

February 01, 2018

DOI:

05:34 min
February 01, 2018

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Summary

Automatically generated

Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.

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