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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
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February 01, 2018
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Summary
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Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.
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Blitz, I. L. Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus. J. Vis. Exp. (132), e56035, doi:10.3791/56035 (2018).
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