É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
8,255 Views
•
05:34 min
•
February 01, 2018
DOI:
Summary
Automatically generated
Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.
Read Article
Cite this Article
Blitz, I. L. Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus. J. Vis. Exp. (132), e56035, doi:10.3791/56035 (2018).
Copy