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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
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JoVE Journal 遺伝学
Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

DOI:
10.3791/56035-v

•

05:34 min

•

February 01, 2018

•

1発生・細胞生物学科,University of California, Irvine

章

  • 00:04標題
  • 00:30Transplantation of PGCs
  • 03:36Post-healing Care of Embryos
  • 04:39Results: CRISPR/Cas9 Leapfrogging in Xenopus
  • 05:16Conclusion

概要

自動翻訳

自動翻訳

Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.

タグ

CRISPR/Cas9LeapfroggingPrimordial Germ Cell TransplantationXenopusDevelopmental GeneticsEssential Genes胚発生Blastula StageVegetal PoleBlastoporeGraft

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