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Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
JoVE Journal
Genetik
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JoVE Journal Genetik
Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

DOI:
10.3791/56035-v

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05:34 min

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February 01, 2018

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1Gelişim ve hücre biyolojisi Anabilim Dalı,Kaliforniya Üniversitesi, Irvine

'Bölümler'

  • 00:04'Başlık'
  • 00:30Transplantation of PGCs
  • 03:36Post-healing Care of Embryos
  • 04:39Results: CRISPR/Cas9 Leapfrogging in Xenopus
  • 05:16Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Genes essential for survival pose technical hurdles for creating mutant lines. Leapfrogging circumvents lethality by combining genome editing with primordial germ cell transplantation to create wild-type animals carrying germline mutations. Leapfrogging also permits the efficient generation of homozygous null mutants in the F1 generation. Here, the transplantation step is demonstrated.

'Etiketler'

CRISPR/Cas9LeapfroggingPrimordial Germ Cell TransplantationXenopusDevelopmental GeneticsEssential GenesEmbriyogenezBlastula StageVegetal PoleBlastoporeGraft

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