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嗅覚神経軸索分子識別コードの視覚化のための嗅球の四重免疫染色
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Neuroscience
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JoVE Journal Neuroscience
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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嗅覚神経軸索分子識別コードの視覚化のための嗅球の四重免疫染色

日本語

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6,982 Views

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06:32 min

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June 05, 2017

DOI:

10.3791/55893-v

DOI:
10.3791/55893-v

•

06:32 min
June 05, 2017

•

7 Views
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1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Summary

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嗅覚ニューロンは、種々の軸索選別分子を発現して適切な神経回路を確立する。このプロトコルは、嗅覚ニューロンの軸索末端における軸索ソーティング分子のコンビナトリアル発現を視覚化する免疫組織化学的染色方法を記載している。

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Ihara, N., Ikegaya, Y., Takeuchi, H. Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes. J. Vis. Exp. (124), e55893, doi:10.3791/55893 (2017).

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