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Immunomarquage quadruple de l'ampoule olfactive pour la visualisation des codes d'identité moléculaire des axes sensoriels olfactifs
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Neuroscience
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JoVE Journal Neuroscience
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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Immunomarquage quadruple de l'ampoule olfactive pour la visualisation des codes d'identité moléculaire des axes sensoriels olfactifs

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6,982 Views

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06:32 min

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June 05, 2017

DOI:

10.3791/55893-v

DOI:
10.3791/55893-v

•

06:32 min
June 05, 2017

•

7 Views
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1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Summary

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Les neurones sensoriels olfactifs expriment une grande variété de molécules de tri d'axones pour établir des circuits neuronaux appropriés. Ce protocole décrit une méthode de coloration immunohistochimique pour visualiser les expressions combinatoires des molécules de tri d'axones aux extrémités axonaires des neurones sensoriels olfactifs.

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Cite this Article

Ihara, N., Ikegaya, Y., Takeuchi, H. Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes. J. Vis. Exp. (124), e55893, doi:10.3791/55893 (2017).

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