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Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
JoVE Journal
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JoVE Journal Nörobilim
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

6,982 Views

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06:32 min

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June 05, 2017

DOI:

10.3791/55893-v

DOI:
10.3791/55893-v

•

06:32 min
June 05, 2017

•

7 Views
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1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

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Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

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Quadruple ImmunostainingOlfactory BulbOlfactory Sensory AxonMolecular Identity CodesAxon Sorting MoleculesOlfactory DevelopmentAxon GuidanceSynapse FormationOlfactory Sensory Neuron RegenerationCryostat SectioningImmunostainingPrimary AntibodiesSecondary Antibodies

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Ihara, N., Ikegaya, Y., Takeuchi, H. Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes. J. Vis. Exp. (124), e55893, doi:10.3791/55893 (2017).

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