Dergi
/
Nörobilim
/
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

DOI:
10.3791/55893-v

•

06:32 min

•

June 05, 2017

•

, ,
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Bölümler

  • 00:05Başlık
  • 01:11Preparation of Parasagittal Olfactory Bulb Sections
  • 02:33Day 1: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:07Day 2: Quadruple Immunostaining of Olfactory Bulb Slices
  • 03:56The Use of Fluorescence Intensity Measurements
  • 04:26Results: Quantification of Axon-sorting Molecules in the Olfactory Bulb
  • 05:42Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

Etiketler

Quadruple ImmunostainingOlfactory BulbOlfactory Sensory AxonMolecular Identity CodesAxon Sorting MoleculesOlfactory DevelopmentAxon GuidanceSynapse FormationOlfactory Sensory Neuron RegenerationCryostat SectioningImmunostainingPrimary AntibodiesSecondary Antibodies

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Analyzing Responses of Mouse Olfactory Sensory Neurons Using the Air-phase Electroolfactogram Recording

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Olfactory Assays for Mouse Models of Neurodegenerative Disease

Ex Vivo Preparations of the Intact Vomeronasal Organ and Accessory Olfactory Bulb

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

Read Article