Journal
/
Neurociência
/
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

6,982 Views

•

06:32 min

•

June 05, 2017

DOI:

10.3791/55893-v

DOI:
10.3791/55893-v

•

06:32 min
June 05, 2017

•

7 Views
, ,
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Summary

Automatically generated

Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

Explore More Videos

Quadruple ImmunostainingOlfactory BulbOlfactory Sensory AxonMolecular Identity CodesAxon Sorting MoleculesOlfactory DevelopmentAxon GuidanceSynapse FormationOlfactory Sensory Neuron RegenerationCryostat SectioningImmunostainingPrimary AntibodiesSecondary Antibodies

Read Article

Cite this Article

Ihara, N., Ikegaya, Y., Takeuchi, H. Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes. J. Vis. Exp. (124), e55893, doi:10.3791/55893 (2017).

Download .ris file

Copy

Share Video

.

Copy