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Viervoudige Immunostaining van de Olfactorische Lamp voor Visualisatie van Olfactorische Sensorische Axon Moleculaire Identiteitscodes
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Neuroscience
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JoVE Journal Neuroscience
Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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Viervoudige Immunostaining van de Olfactorische Lamp voor Visualisatie van Olfactorische Sensorische Axon Moleculaire Identiteitscodes

Nederlands

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6,982 Views

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06:32 min

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June 05, 2017

DOI:

10.3791/55893-v

DOI:
10.3791/55893-v

•

06:32 min
June 05, 2017

•

7 Views
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1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Summary

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Olfactorische zintuiglijke neuronen vormen een grote verscheidenheid aan axon-sorterende moleculen om een ​​goede neurale schakeling te vormen. Dit protocol beschrijft een immunohistochemische kleuring methode om combinatorische expressies van axon-sorterende moleculen te visualiseren bij de axon termini van olfactorische sensorische neuronen.

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Ihara, N., Ikegaya, Y., Takeuchi, H. Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes. J. Vis. Exp. (124), e55893, doi:10.3791/55893 (2017).

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