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Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
Chapters
- 00:05Title
- 01:11Preparation of Parasagittal Olfactory Bulb Sections
- 02:33Day 1: Quadruple Immunostaining of Olfactory Bulb Slices
- 03:07Day 2: Quadruple Immunostaining of Olfactory Bulb Slices
- 03:56The Use of Fluorescence Intensity Measurements
- 04:26Results: Quantification of Axon-sorting Molecules in the Olfactory Bulb
- 05:42Conclusion
Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.
