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用于炎症反应的新内皮调节因子的筛选测定
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Immunology and Infection
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JoVE Journal Immunology and Infection
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
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用于炎症反应的新内皮调节因子的筛选测定

DOI:
10.3791/55824-v

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12:50 min

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September 15, 2017

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, , , ,
1Pharmacological Therapies Unit, Research Institute for Rare Diseases,Institute of Health Carlos III

Chapters

  • 00:05Title
  • 00:58Endothelial Cell Culture and Tissue Culture Conditions
  • 02:42Evaluate Endothelial Factor Released on Leukocyte Activation by Adhesion Assays
  • 04:12Leukocyte Adhesion Assay
  • 07:42Test Endothelial Activation by Leukocyte Endothelium Co-adhesion Assay
  • 09:59Results: Activation Parameters of LPS-stimulated Endothelial Cells and Influence on Leukocyte Behavior
  • 11:52Conclusion

Summary

Automatic Translation

Automatic Translation

血管内皮严密控制白细胞的招募。不充分的白细胞外渗导致人类炎症性疾病。因此, 寻找新的调节元素内皮活化是必要的设计改进治疗炎症性疾病。在这里, 我们描述了一个全面的方法来表征新的内皮调节剂, 可以改变白细胞贩运在炎症。

Tags

Endothelial RegulatorsInflammatory ResponseScreening AssayInflammatory DisordersLeukocyte RecruitmentCytokinesChemokinesAdhesion MoleculesRT-qPCRWesMLEC-04 CellsLipopolysaccharide (LPS)Ccl5Cxcl1Cxcl10E-selectinVCAM-1ICAM-1

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