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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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March 08, 2012
DOI:
1Department of Microbiology and Parasitology, College of Basic Medical Sciences,China Medical University , 2Department of Obstetrics and Gynecology, College of Medicine,University of Arkansas for Medical Sciences , 3Department of Pathology, College of Medicine,University of Arkansas for Medical Sciences
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Interferon-gammaEnzyme-linked Immunospot AssayT-cell EpitopesHuman Papillomavirus (HPV)ProtocolIsolating And Characterizing Rare T CellsHPV ProteinsPeptide-specific T CellsLoop Electrical Excision ProcedureHigh-grade Squamous Intraepithelial LesionHuman Papillomavirus Type 16 (HPV 16)E6 And E7 ProteinsIFN-g Enzyme-linked Immunospot (ELISPOT) AssaySynthetic PeptidesEpitope Specific T CellsCD8 T-cell Isolation KitMiltenyi Biotec
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Wang, X., Greenfield, W. W., Coleman, H. N., James, L. E., Nakagawa, M. Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus. J. Vis. Exp. (61), e3657, doi:10.3791/3657 (2012).
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