Journal
/
Immunology and Infection
/
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

English

Automatically Generated

8,104 Views

•

10:39 min

•

November 27, 2014

DOI:

10.3791/52121-v

DOI:
10.3791/52121-v

•

10:39 min
November 27, 2014

•

4 Views
, , , ,
1Department of Microbiology & Immunology,The University of Texas Medical Branch, 2Department of Pathology,The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development,The University of Texas Medical Branch

Summary

Automatically generated

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

Explore More Videos

West Nile VirusWNVNS4B-P38G MutantMyd88T Cell ResponseDendritic CellsIn Vitro T Cell Priming AssayIntracellular Cytokine StainingCFSEOTII Transgenic MiceMicrocentrifuge Tube SystemBiosafety Level 3

Read Article

Cite this Article

Xie, G., Whiteman, M. C., Wicker, J. A., Barrett, A. D., Wang, T. In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection. J. Vis. Exp. (93), e52121, doi:10.3791/52121 (2014).

Download .ris file

Copy

Share Video

.

Copy