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La inducción de una respuesta inflamatoria en la Primaria hepatocitos culturas de ratones
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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La inducción de una respuesta inflamatoria en la Primaria hepatocitos culturas de ratones

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9,773 Views

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08:32 min

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March 10, 2017

DOI:

10.3791/55319-v

DOI:
10.3791/55319-v

•

08:32 min
March 10, 2017

•

3 Views
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1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Summary

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Aquí, se muestra un enfoque enzimático para aislar hepatocitos primarios de ratones adultos, y se describe la cuantificación de una respuesta inflamatoria mediante ELISA y PCR en tiempo real.

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Cite this Article

Czaya, B., Singh, S., Yanucil, C., Schramm, K., Faul, C., Grabner, A. Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice. J. Vis. Exp. (121), e55319, doi:10.3791/55319 (2017).

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