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Immunology and Infection

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マウスからの初代肝細胞培養における炎症反応の誘導

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

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マウスからの初代肝細胞培養における炎症反応の誘導

9,773 Views

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08:32 min

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March 10, 2017

DOI:

10.3791/55319-v

DOI:

10.3791/55319-v

•

08:32 min

March 10, 2017

•

3 Views

Brian Czaya*^1,2, Saurav Singh*^1,2, Christopher Yanucil^1,2, Karla Schramm^1,2, Christian Faul^1,2, Alexander Grabner¹

¹Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, ²Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Summary

ここでは、成体マウスからの初代肝細胞を単離するための酵素的アプローチを示し、我々は、ELISAおよびリアルタイムPCRを使用して炎症反応の定量化を記載します。

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