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Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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JoVE Journal 免疫学と感染
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

9,773 Views

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08:32 min

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March 10, 2017

DOI:

10.3791/55319-v

DOI:
10.3791/55319-v

•

08:32 min
March 10, 2017

•

3 Views
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1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

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Here, we show an enzymatic approach to isolate primary hepatocytes from adult mice, and we describe the quantification of an inflammatory response using ELISA and real-time PCR.

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Cite this Article

Czaya, B., Singh, S., Yanucil, C., Schramm, K., Faul, C., Grabner, A. Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice. J. Vis. Exp. (121), e55319, doi:10.3791/55319 (2017).

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