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Die Induktion einer entzündlichen Reaktion in der Primär Hepatozyten-Kulturen von Mäusen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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Die Induktion einer entzündlichen Reaktion in der Primär Hepatozyten-Kulturen von Mäusen

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9,773 Views

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08:32 min

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March 10, 2017

DOI:

10.3791/55319-v

DOI:
10.3791/55319-v

•

08:32 min
March 10, 2017

•

3 Views
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1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Summary

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Hier zeigen wir eine enzymatische Annäherung an primären Hepatozyten aus erwachsenen Mäusen zu isolieren, und wir beschreiben die Quantifizierung einer Entzündungsreaktion mittels ELISA und real-time PCR.

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Cite this Article

Czaya, B., Singh, S., Yanucil, C., Schramm, K., Faul, C., Grabner, A. Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice. J. Vis. Exp. (121), e55319, doi:10.3791/55319 (2017).

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