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L'induction d'une réponse inflammatoire dans les cultures hépatocytes primaires de souris
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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L'induction d'une réponse inflammatoire dans les cultures hépatocytes primaires de souris

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9,773 Views

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08:32 min

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March 10, 2017

DOI:

10.3791/55319-v

DOI:
10.3791/55319-v

•

08:32 min
March 10, 2017

•

3 Views
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1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Summary

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Ici, nous montrons une approche enzymatique pour isoler des hépatocytes primaires de souris adultes, et nous décrivons la quantification d'une réponse inflammatoire par ELISA et la PCR en temps réel.

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Cite this Article

Czaya, B., Singh, S., Yanucil, C., Schramm, K., Faul, C., Grabner, A. Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice. J. Vis. Exp. (121), e55319, doi:10.3791/55319 (2017).

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