Rivista
/
Immunologia e infezioni
/
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

9,773 Views

•

08:32 min

•

March 10, 2017

DOI:

10.3791/55319-v

DOI:
10.3791/55319-v

•

08:32 min
March 10, 2017

•

3 Views
, , , , ,
1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Summary

Automatically generated

Here, we show an enzymatic approach to isolate primary hepatocytes from adult mice, and we describe the quantification of an inflammatory response using ELISA and real-time PCR.

Explore More Videos

HepatocytesInflammatory ResponseCytokinesCRPIL6Hepatic Acute Phase ResponseHepatocellular FunctionHepatic CytokinesChronic Kidney DiseaseHepatic MetabolomicsInflammationLiver PerfusionLiver DigestIVC CannulationLiver Dissection

Read Article

Cite this Article

Czaya, B., Singh, S., Yanucil, C., Schramm, K., Faul, C., Grabner, A. Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice. J. Vis. Exp. (121), e55319, doi:10.3791/55319 (2017).

Download .ris file

Copy

Share Video

.

Copy