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Journal
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Developmental Biology
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在Active ERK的时空分析<em>℃。线虫</em>生殖系
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Spatial and Temporal Analysis of Active ERK in the
C. elegans
Germline
Please note that all translations are automatically generated.
Click here for the English version.
在Active ERK的时空分析<em>℃。线虫</em>生殖系
DOI:
10.3791/54901-v
•
08:40 min
•
November 29, 2016
•
Amanda L. Gervaise
,
Swathi Arur
2
1
Program in Developmental Biology
,
Baylor College of Medicine
,
2
Department of Genetics
,
UT MD Anderson Cancer Center
Chapters
00:05
Title
00:35
Dissection of Adult Worms for Obtaining Gonads
02:21
Gonad Fixation
04:22
Blocking and Antibody-staining
06:18
Mounting the Tissue
07:15
Results: ERK Activity in Germline Tissues
07:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们目前在解剖
C.
积极ERK的时间和空间定位的免疫基于成像的方法
线虫
性腺。这里所描述的协议可以适于任何信令的可视化或结构蛋白中的
C.线虫
性腺,提供了合适的抗体试剂是可用的。
Tags
ERK Signaling
C. Elegans
Germline
In Vivo
Quantification
Spatial And Temporal Analysis
Drug Treatments
Gene Perturbations
Dissection
Immunofluorescence
Microscopy
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