Journal
/
/
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

9,823 Views

08:40 min

November 29, 2016

DOI:

08:40 min
November 29, 2016

11 Views
,

Summary

Automatically generated

We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.

Read Article