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Aktif ERK Mekansal ve Zamansal Analizi<em> C. elegans</em> Germline
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
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Aktif ERK Mekansal ve Zamansal Analizi<em> C. elegans</em> Germline

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9,871 Views

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08:40 min

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November 29, 2016

DOI:

10.3791/54901-v

DOI:
10.3791/54901-v

•

08:40 min
November 29, 2016

•

11 Views
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1Program in Developmental Biology,Baylor College of Medicine, 2Department of Genetics,UT MD Anderson Cancer Center

Summary

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Biz disseke C. aktif ERK mekansal ve zamansal lokalizasyonu için immünofloresan görüntüleme bazlı yöntem mevcut elegans gonad. Burada açıklanan protokol C herhangi bir sinyal görüntülenmesi veya yapısal protein için uyarlanabilir elegans yumurtalık, uygun bir antikor reaktifi kullanılabilir sağladı.

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Cite this Article

Gervaise, A. L., Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (117), e54901, doi:10.3791/54901 (2016).

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