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Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
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Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
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November 29, 2016
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We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.
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Gervaise, A. L., Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (117), e54901, doi:10.3791/54901 (2016).
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